A RAT PITUITARY-TUMOR CELL-LINE (GH(3)) EXPRESSES TYPE-I AND TYPE-II RECEPTORS AND OTHER CELL-SURFACE BINDING PROTEIN(S) FOR TRANSFORMING GROWTH-FACTOR-BETA

被引：20

作者：

YAMASHITA, H ^{[1
]}

OKADOME, T ^{[1
]}

FRANZEN, P ^{[1
]}

TENDIJKE, P ^{[1
]}

HELDIN, CH ^{[1
]}

MIYAZONO, K ^{[1
]}

机构：

[1] LUDWIG INST CANC RES,CTR BIOMED,S-75124 UPPSALA,SWEDEN

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1995年 / 270卷 / 02期

关键词：

D O I：

10.1074/jbc.270.2.770

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A rat pituitary tumor cell line (GH(3)) has been reported to express transforming grow-th factor-beta (TGF-beta) binding components of 70-74 kDa (ligand included), denoted TGF-beta type IV receptor. We investigated whether the type IV receptor corresponds to any of the recently cloned type I receptors for proteins in the TGF-beta superfamily. TGF-beta type I receptor (T beta R-I) complexes of 69-72 kDa formed a heteromeric complex with T beta R-II in GH(3) cells, as detected by immunoprecipitation. In addition, TGF-beta formed complexes of 72-74 kDa, which were different from T beta R-I and the other known type I receptors, and were not dependent on T beta R-II for binding, The GH(3) cells were resistant to the growth inhibitory activity of TGF-beta, but a transcriptional response was activated by TGF-beta in this cell line, presumably through the T beta R-II and T beta R-I complex. These results indicate that GH(3) cells have T beta R-I and T beta R-II and, in addition, other binding protein(s) which form 72-74-kDa complexes with TGF-beta; the function of the latter component(s) remains to be elucidated.

引用

页码：770 / 774

页数：5

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