INTRACELLULAR CALCIUM IN THE CONTROL OF OSTEOCLAST FUNCTION .2. PARADOXICAL ELEVATION OF CYTOSOLIC FREE CALCIUM BY VERAPAMIL

被引：35

作者：

ZAIDI, M

MACINTYRE, I

DATTA, H

机构：

[1] Cell Biology Unit, Royal Postgraduate Medical School, London

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0006-291X(90)92097-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here for the first time that verapamil elevates cytosolic calcium. We have found that in the isolated rat osteoclast, verapamil at low micromolar concentrations did not block the elevation of cytosolic free calcium ([Ca++]i) in response to elevated extracellular calcium concentrations ([Ca++]e). However, high micromolar concentrations of verapamil (300 μM or above) led to a rapid sustained elevation of [Ca++]i. These concentrations of verapamil had effects on osteoclast morphology and resorptive activity that were similar to those produced by elevated [Ca++]e: there was a marked dose-dependent fall in cell spread area and osteoclastic bone resorption. The sensitivity of osteoclasts to high micromolar concentrations of verapamil is unique and could not be mimicked in macrophages and lymphocytes. © 1990.

引用

页码：807 / 812

页数：6

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