ISOLATION OF HUMAN GERM-LINE DNA SUITABLE FOR RECOMBINANT DNA STUDIES

被引：4

作者：

WALLACE, BE

SALSER, W

机构：

[1] UNIV CALIF LOS ANGELES,DEPT BIOL,LOS ANGELES,CA 90024

[2] UNIV CALIF LOS ANGELES,INST MOLEC BIOL,LOS ANGELES,CA 90024

来源：

GENE | 1979年 / 7卷 / 3-4期

关键词：

CsCl gradient; P2-EK2; biocontainment; Sperm DNA cloning;

D O I：

10.1016/0378-1119(79)90053-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

DNA from human sperm cells can be isolated with size and purity sufficient for use in recombinant DNA research. The DNA averages 100 000 base pairs (bp) in size (about 70·106 daltons) and is free of somatic cell, bacterial, and viral DNA. It can therefore be cloned under P2+EK2 conditions as stipulated in the 1978 NIH Guidelines for recombinant DNA research. © 1979.

引用

页码：343 / 347

页数：5

共 7 条

[1] ISOLATION AND PROPERTIES OF DEOXYRIBONUCLEIC ACID FROM MAMMALIAN SPERM
BORENFREUND, E
BENDICH, A
FITT, E
[J]. NATURE, 1961, 191 (479) : 1375 - &
[2] SEDIMENTATION RATE AS A MEASURE OF MOLECULAR WEIGHT OF DNA
BURGI, E
HERSHEY, AD
[J]. BIOPHYSICAL JOURNAL, 1963, 3 (04) : 309 - &
[3] ISOLATION OF HIGH-MOLECULAR-WEIGHT DNA FROM MAMMALIAN-CELLS
GROSS-BELLARD, MM
OUDET, P
CHAMBON, P
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1973, 36 (01): : 32 - 38
[4] SOBER HA, 1970, HDB BIOCH, pH113
[5] SEDIMENTATION STUDIES OF SIZE AND SHAPE OF DNA
STUDIER, FW
[J]. JOURNAL OF MOLECULAR BIOLOGY, 1965, 11 (02) : 373 - &
[6] WITKIN S, 1965, P NATL ACAD SCI US, V72, P3295
[7] 1978, FED REG 60108, V43

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