PURIFICATION OF THE INSECTICIDAL TOXIN FROM THE PARASPORAL CRYSTAL OF BACILLUS-THURINGIENSIS SUBSP KURSTAKI

被引：40

作者：

BULLA, LA

DAVIDSON, LI

KRAMER, KJ

JONES, BL

机构：

[1] U.S. Grain Marketing Research Laboratory, Agricultural Research, Science and Education Administration, Manhattan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91997-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The insecticidal toxin of Bacillus thuringiensis subsp. kurstaki was isolated from parasporal crystals. The toxin, which is stable for several months, is a glycoprotein with an apparent molecular weight of 68,000 that is generated upon solubilization and activation of a higher molecular weight protoxin (MWapp = 1.3 × 105) at alkaline pH. The toxin was purified by gel filtation and anion exchange chromatography and its molecular weight was established by gel filtration chromatography and SDS polyacrylamide gel electrophoresis. © 1979.

引用

页码：1123 / 1130

页数：8

共 14 条

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[14]

Weber K, 1972, Methods Enzymol, V26, P3

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