PHOSPHORYLATION AND ACTIVATION OF HORMONE-SENSITIVE LIPASE IN ISOLATED MACROPHAGES

被引：44

作者：

SMALL, CA

ROGERS, MP

GOODACRE, JA

YEAMAN, SJ

机构：

[1] UNIV NEWCASTLE UPON TYNE,SCH MED,DEPT BIOCHEM & GENET,NEWCASTLE TYNE NE2 4HH,ENGLAND

[2] UNIV NEWCASTLE UPON TYNE,SCH MED,DEPT RHEUMATOL,NEWCASTLE TYNE NE2 4HH,ENGLAND

来源：

FEBS LETTERS | 1991年 / 279卷 / 02期

关键词：

HORMONE-SENSITIVE LIPASE; PROTEIN PHOSPHORYLATION; MACROPHAGE; CHOLESTEROL METABOLISM; VARIATE NORMAL DISTRIBUTIONS;

D O I：

10.1016/0014-5793(91)80179-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hormone-sensitive lipase (HSL) is responsible for the neutral cholesterol ester hydrolase activity in macrophages. Incubation of intact WEHI macrophages or mouse peritoneal macrophages leads to phosphorylation of HSL, which is increased by incubation with either dibutyryl cyclic AMP and 3-isobutyl-1-methylxanthine or okadaic acid. Correspondingly, these agents also activate neutral cholesterol ester hydrolase activity in intact WEHI cells. Regulation of mobilisation of esterified cholesterol in macrophages may be of antiatherogenic value, which this model system now allows us to investigate further.

引用

页码：323 / 326

页数：4

共 14 条

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BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

BROWN MS, 1980, J BIOL CHEM, V255, P9344

[3] OKADAIC ACID - A NEW PROBE FOR THE STUDY OF CELLULAR-REGULATION [J].