FISSION YEAST PROMOTER-PROBE VECTORS BASED ON HYGROMYCIN RESISTANCE

被引：14

作者：

BURLAND, TG ^{[1
]}

PALLOTTA, D ^{[1
]}

TARDIF, MC ^{[1
]}

LEMIEUX, G ^{[1
]}

DOVE, WF ^{[1
]}

机构：

[1] UNIV LAVAL,FAC SCI & ENGN,DEPT BIOL,QUEBEC CITY G1K 7P4,QUEBEC,CANADA

来源：

GENE | 1991年 / 100卷

关键词：

RECOMBINANT DNA; PLASMID; DNA TRANSFORMATION; SCHIZOSACCHAROMYCES-POMBE; SACCHAROMYCES-CEREVISIAE; SLIME MOLD; PHYSARUM;

D O I：

10.1016/0378-1119(91)90374-K

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have constructed fission yeast vectors that carry either compelte or 5'-truncated alleles of the hph gene, encoding hygromycin B phosphotransferase. We show that plasmid-borne hph can be expressed in fission yeast to confer hygromycin resistance. The vectors permit selection or screening in fission yeast for promoter activity of DNA fragments from other species. We used the vectors to identify several genomic sequences from Physarum that provide promoter function in fission yeast.

引用

页码：241 / 245

页数：5

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