CORRELATION BETWEEN LACK OF BONE GLA PROTEIN MESSENGER-RNA EXPRESSION IN RAT TRANSPLANTABLE OSTEOSARCOMAS AND EXPRESSION OF BOTH C-FOS AND C-JUN PROTOONCOGENES

被引:9
作者
HONOKI, K
DOHI, Y
TABATA, S
MII, Y
MIYAUCHI, Y
TSUTSUMI, M
TSUJIUCHI, T
MORISHITA, T
MIURA, S
MORIYAMA, T
TAMAI, S
KONISHI, Y
机构
[1] NARA MED UNIV,DEPT ONCOL PATHOL,840 SHIJO,KASHIHARA,NARA 634,JAPAN
[2] NARA MED UNIV,DEPT ORTHOPED SURG,KASHIHARA,NARA 634,JAPAN
[3] NARA MED UNIV,DEPT PUBL HLTH,KASHIHARA,NARA 634,JAPAN
[4] NARA MED UNIV,DEPT CHEM,KASHIHARA,NARA 634,JAPAN
关键词
OSTEOSARCOMA; ALKALINE PHOSPHATASE; BONE GLA PROTEIN; C-FOS; C-JUN;
D O I
10.1002/mc.2940070209
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Alkaline phosphatase (AP) activity and expression of bone Gla protein (BGP), c-fos, and c-jun were compared in two transplantable osteosarcomas with high potentials for metastasis to the lung. The original spontaneous osteosarcoma (SOS) gradually became histologically undifferentiated, losing its osteogenic activity during serial transfer, whereas the chemical (4-hydroxyaminoquinoline 1-oxide)-induced osteosarcoma (COS) retained osteogenesis. The two osteosarcomas showed similar doubling times and levels of lung metastasis, and strong AP activity was detected on the cell membranes of both. Northern blot analysis revealed that lack of BGP mRNA expression was associated with expression of both c-fos and c-jun proto-oncogenes in SOS. In contrast, neither c-fos nor c-jun mRNAs were detected but BGP mRNA was expressed in the case of COS. These results suggest that the c-fos and c-jun genes may suppress the expression of BGP mRNA relevant to differentiation and osteoid formation in rat osteosarcomas. However, this does not appear to be directly related to proliferative or metastatic biological behavior.
引用
收藏
页码:111 / 115
页数:5
相关论文
共 28 条
[1]   FACTORS THAT PROMOTE PROGRESSIVE DEVELOPMENT OF THE OSTEOBLAST PHENOTYPE IN CULTURED FETAL-RAT CALVARIA CELLS [J].
ARONOW, MA ;
GERSTENFELD, LC ;
OWEN, TA ;
TASSINARI, MS ;
STEIN, GS ;
LIAN, JB .
JOURNAL OF CELLULAR PHYSIOLOGY, 1990, 143 (02) :213-221
[2]  
BRONCKERS ALJJ, 1987, BONE MINER, V2, P361
[3]   A METHOD FOR ISOLATION OF INTACT, TRANSLATIONALLY ACTIVE RIBONUCLEIC-ACID [J].
CATHALA, G ;
SAVOURET, JF ;
MENDEZ, B ;
WEST, BL ;
KARIN, M ;
MARTIAL, JA ;
BAXTER, JD .
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1983, 2 (04) :329-335
[4]  
DOHI Y, IN PRESS J BONE MINE
[5]   PROTOONCOGENE C-FOS EXPRESSION IN GROWTH REGIONS OF FETAL BONE AND MESODERMAL WEB TISSUE [J].
DONY, C ;
GRUSS, P .
NATURE, 1987, 328 (6132) :711-714
[6]  
FRANSEEN CLIFFORD C., 1935, AMER JOUR CANCER, V24, P299
[7]   EXPRESSION OF THE TRANSIN, C-FOS, AND C-JUN GENES IN RAT TRANSPLANTABLE OSTEOSARCOMAS AND MALIGNANT FIBROUS HISTIOCYTOMAS [J].
HONOKI, K ;
TSUTSUMI, M ;
TSUJIUCHI, T ;
KONDOH, S ;
SHIRAIWA, K ;
MIYAUCHI, Y ;
MII, Y ;
TAMAI, S ;
KONISHI, Y ;
BOWDEN, GT .
MOLECULAR CARCINOGENESIS, 1992, 6 (02) :122-128
[8]  
JEFREE GM, 1965, J BONE JOINT SURG, V47, P120
[9]  
KONISHI Y, 1984, AM J PATHOL, V115, P469
[10]  
KONISHI Y, 1982, J NATL CANCER I, V68, P859