EFFECTS OF NERVE GROWTH-FACTOR AND BASIC FIBROBLAST GROWTH-FACTOR ON SURVIVAL OF CULTURED SEPTAL CHOLINERGIC NEURONS FROM ADULT-RATS

被引：32

作者：

KUSHIMA, Y ^{[1
]}

NISHIO, C ^{[1
]}

NONOMURA, T ^{[1
]}

HATANAKA, H ^{[1
]}

机构：

[1] OSAKA UNIV,INST PROT RES,DIV PROT BIOSYNTH,3-2 YAMADAOKA,SUITA,OSAKA 565,JAPAN

来源：

BRAIN RESEARCH | 1992年 / 598卷 / 1-2期

关键词：

NEUROTROPHIC FACTOR; SEPTUM; ACETYLCHOLINESTERASE; NEURONAL CELL SURVIVAL; NEURONAL CELL CULTURE; PAPAIN DIGESTION;

D O I：

10.1016/0006-8993(92)90192-C

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have established a Primary culture technique for neuronal cells from rat basal forebrain from postnatal day 58 (P58) to study the effects of neurotrophic factors on the neurons. The survival of acetylcholinesterase (AChE)-positive neurons of 2-week-old rat septum has already been reported to be strongly supported by nerve growth factor (NGF) in culture. In this culture study of neurons from adult rat brains, the survival of AChE-positive neurons from P58 rat septum was slightly improved by NGF, although low affinity NGF receptor expression was also observed on cultured P58 rat septum neurons as well as on those from 2-week-old rats. The addition of basic fibroblast growth factor (bFGF) improved the survival of AChE-positive neurons cultured from P58 rat septum, but did not promote the survival of neurons from P12 rat septum. These results suggest that NGF changes to a maintenance factor in adult rat brain from a survival factor in postnatal 2-week-old rats. The survival of cholinergic neurons in culture of adult rat septum might be supported by factor(s) other than NGF, such as bFGF.

引用

页码：264 / 270

页数：7

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