SIMULTANEOUS IDENTIFICATION OF STRAINS OF ESCHERICHIA-COLI SEROTYPE O157-H7 AND THEIR SHIGA-LIKE TOXIN TYPE BY MISMATCH AMPLIFICATION MUTATION ASSAY-MULTIPLEX PCR

被引：335

作者：

CEBULA, TA ^{[1
]}

PAYNE, WL ^{[1
]}

FENG, P ^{[1
]}

机构：

[1] US FDA,CTR FOOD SAFETY & APPL NUTR,WASHINGTON,DC 20204

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 01期

关键词：

D O I：

10.1128/JCM.33.1.248-250.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mismatch amplification mutation assay primers, specific for a unique base substitution in uidA of Escherichia call O157:H7, was coupled with primers for the Shiga-like toxin I (SLT-I) and SLT-II genes in a multiplex PCR assay. Analysis of 108 bacteria showed that all Escherichia coli serotype O157:H7 strains were identified simultaneously with the SLT types encoded by these strains.

引用

页码：248 / 250

页数：3

共 18 条

[1] ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR DETECTING ANTIBODIES TO SHIGA-LIKE TOXIN-I, SHIGA-LIKE TOXIN-II, AND ESCHERICHIA-COLI O157-H7 LIPOPOLYSACCHARIDE IN HUMAN SERUM
BARRETT, TJ
GREEN, JH
GRIFFIN, PM
PAVIA, AT
OSTROFF, SM
WACHSMUTH, IK
[J]. CURRENT MICROBIOLOGY, 1991, 23 (04) : 189 - 195
[2] ISOLATION OF A CITROBACTER-FREUNDII STRAIN WHICH CARRIES THE ESCHERICHIA-COLI O157 ANTIGEN
BETTELHEIM, KA
EVANGELIDIS, H
PEARCE, JL
SOWERS, E
STROCKBINE, NA
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (03) : 760 - 761
[3] BOKETE TN, 1993, GASTROENTEROLOGY, V105, P1724, DOI 10.1016/0016-5085(93)91069-T
[4] Cha R S, 1992, PCR Methods Appl, V2, P14
[5] IDENTIFICATION OF UIDA GENE-SEQUENCES IN BETA-D-GLUCURONIDASE-NEGATIVE ESCHERICHIA-COLI
FENG, P
LUM, R
CHANG, GW
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (01) : 320 - 323
[6] IDENTIFICATION OF ESCHERICHIA-COLI SEROTYPE O157-H7 BY DNA PROBE SPECIFIC FOR AN ALLELE OF UID A-GENE
FENG, P
[J]. MOLECULAR AND CELLULAR PROBES, 1993, 7 (02) : 151 - 154
[7] GENETIC-ANALYSIS OF UIDA EXPRESSION IN ENTEROHEMORRHAGIC ESCHERICHIA-COLI SEROTYPE O157-H7
FENG, P
LAMPEL, KA
[J]. MICROBIOLOGY-SGM, 1994, 140 : 2101 - 2107
[8] VIRULENCE OF AN ESCHERICHIA-COLI O157-H7 SORBITOL-POSITIVE MUTANT
FRATAMICO, PM
BUCHANAN, RL
COOKE, PH
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (12) : 4245 - 4252
[9] THE EPIDEMIOLOGY OF INFECTIONS CAUSED BY ESCHERICHIA-COLI O157-H7, OTHER ENTEROHEMORRHAGIC ESCHERICHIA-COLI, AND THE ASSOCIATED HEMOLYTIC UREMIC SYNDROME
GRIFFIN, PM
TAUXE, RV
[J]. EPIDEMIOLOGIC REVIEWS, 1991, 13 : 60 - 98
[10] MOLECULAR-DETECTION OF SORBITOL-FERMENTING ESCHERICHIA-COLI O157 IN PATIENTS WITH HEMOLYTIC-UREMIC SYNDROME
GUNZER, F
BOHM, H
RUSSMANN, H
BITZAN, M
ALEKSIC, S
KARCH, H
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) : 1807 - 1810

← 1 2 →