ANALYSIS OF ALPHA-LINOLENIC ACID GEOMETRICAL-ISOMERS IN DEODORIZED OILS BY CAPILLARY GAS-LIQUID-CHROMATOGRAPHY ON CYANOALKYL POLYSILOXANE STATIONARY PHASES - A NOTE OF CAUTION

被引：45

作者：

WOLFF, RL

机构：

[1] ISTAB, Laboratoire de Lipochimie Alimentaire, Universite Bordeaux 1, Talence Cedex, 33405, Allee des Facultes

来源：

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY | 1994年 / 71卷 / 08期

关键词：

ALPHA-LINOLENIC ACID; CYANOALKYL POLYSILOXANE STATIONARY PHASE; DEODORIZED OILS; EQUIVALENT CHAINLENGTH; FATTY ACID METHYL ESTERS; GAS-LIQUID CHROMATOGRAPHY; GEOMETRICAL ISOMERS; TEMPERATURE EFFECTS; TRANS FATTY ACIDS;

D O I：

10.1007/BF02540472

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Analysis of alpha-linolenic acid geometrical isomers in deodorized or heated oils by capillary gas-liquid chromatography (GLC) on polar cyanoalkyl polysiloxane stationary phases requires some care to avoid interferences with other fatty acids. Depending on the temperature of the column, the cis-11 20:1 acid may elute before, with or after the cis-9,cis-12,cis-15 18:3 acid during GLC. In some instances [temperature higher than 180 degrees C with a CP Sil 88 column (Chrompack, Middelburg, The Netherlands)], the 20:1 acid coelutes with the trans-9,cis-12,cis-15 18:3 acid, leading to abnormally high levels of this last isomer. Consequently, the degree of isomerization of alpha-linolenic acid will be overestimated under such conditions. It is recommended that the behavior of cis-11 20:1 acid relative to temperature be checked carefully prior to the determination of alpha-linolenic acid geometrical isomers by GLC. Temperatures lower than 160 degrees C seem appropriate to separate all of these components from each other and from cis-11 20:1 acid in a 50 m X 0.25 mm i.d. CP Sil 88 capillary column.

引用

页码：907 / 909

页数：3

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