INTERACTION BETWEEN THE ACCEPTOR END OF TRANSFER-RNA AND THE T-BOX STIMULATES ANTITERMINATION IN THE BACILLUS-SUBTILIS TYRS GENE - A NEW ROLE FOR THE DISCRIMINATOR BASE

被引:87
作者
GRUNDY, FJ [1 ]
ROLLINS, SM [1 ]
HENKIN, TM [1 ]
机构
[1] ALBANY MED COLL,DEPT BIOCHEM & MOLEC BIOL,ALBANY,NY 12208
关键词
D O I
10.1128/JB.176.15.4518-4526.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Bacillus subtilis tyrS gene is a member of a group of gram-positive aminoacyl-tRNA synthetase and amino acid biosynthesis genes which are regulated by transcription antitermination. Each gene in the group is specifically induced by limitation for the appropriate amino acid. This response is mediated by interaction of the cognate tRNA with the mRNA leader region to promote formation of an antiterminator structure. The tRNA interacts with the leader by codon-anticodon pairing at a position designated the specifier sequence which is upstream of the antiterminator. In this study, an additional site of possible contact between the tRNA and the leader was identified through covariation of leader mRNA and tRNA sequences. Mutations in the acceptor end of tRNA(Tyr) could suppress mutations in the side bulge of the antiterminator, in a pattern consistent with base pairing. This base pairing may thereby directly affect the formation and/or function of the antiterminator. The discriminator position of the tRNA, an important identity determinant for a number of tRNAs, including tRNA(Tyr), was shown to act as a second specificity determinant for assuring response to the appropriate tRNA. Furthermore, overproduction of an unchargeable variant of tRNA(Tyr) resulted in antitermination in the absence of limitation for tyrosine, supporting the proposal that uncharged tRNA is the effector in this system.
引用
收藏
页码:4518 / 4526
页数:9
相关论文
共 43 条
[1]   REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS [J].
ANAGNOSTOPOULOS, C ;
SPIZIZEN, J .
JOURNAL OF BACTERIOLOGY, 1961, 81 (05) :741-&
[2]  
CHOW KC, 1988, GENE, V73, P537
[3]   IS THERE A DISCRIMINATOR SITE IN TRANSFER-RNA [J].
CROTHERS, DM ;
SENO, T ;
SOLL, DG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (10) :3063-+
[4]   THE SACT GENE REGULATING THE SACPA OPERON IN BACILLUS-SUBTILIS SHARES STRONG HOMOLOGY WITH TRANSCRIPTIONAL ANTITERMINATORS [J].
DEBARBOUILLE, M ;
ARNAUD, M ;
FOUET, A ;
KLIER, A ;
RAPOPORT, G .
JOURNAL OF BACTERIOLOGY, 1990, 172 (07) :3966-3973
[5]  
Ehrlich S., COMMUNICATION
[6]  
GAGNON Y, 1994, J BIOL CHEM, V269, P7473
[7]  
GARRITY DB, COMMUNICATION
[8]   EXPRESSION OF BOTH BACILLUS-SUBTILIS THREONYL-TRANSFER-RNA SYNTHETASE GENES IS AUTOGENOUSLY REGULATED [J].
GENDRON, N ;
PUTZER, H ;
GRUNBERGMANAGO, M .
JOURNAL OF BACTERIOLOGY, 1994, 176 (02) :486-494
[9]   BRANCHED-CHAIN AMINO-ACID BIOSYNTHESIS GENES IN LACTOCOCCUS-LACTIS SUBSP LACTIS [J].
GODON, JJ ;
CHOPIN, MC ;
EHRLICH, SD .
JOURNAL OF BACTERIOLOGY, 1992, 174 (20) :6580-6589
[10]   REGULATION OF THE BACILLUS-SUBTILIS TRP OPERON BY AN RNA-BINDING PROTEIN [J].
GOLLNICK, P .
MOLECULAR MICROBIOLOGY, 1994, 11 (06) :991-997