HIV-1 COAT PROTEIN GP120-INDUCED INCREASES IN LEVELS OF INTRASYNAPTOSOMAL CALCIUM

被引：34

作者：

NATH, A

PADUA, RA

GEIGER, JD

机构：

[1] UNIV MANITOBA,FAC MED,DEPT PHYSIOL,WINNIPEG,MB R3E 0W3,CANADA

[2] UNIV MANITOBA,FAC MED,DEPT MED MICROBIOL,WINNIPEG,MB R3E 0W3,CANADA

[3] UNIV MANITOBA,FAC MED,DEPT INTERNAL MED,NEUROL SECT,WINNIPEG,MB R3E 0W3,CANADA

来源：

BRAIN RESEARCH | 1995年 / 678卷 / 1-2期

关键词：

HIV-1; GP120; CALCIUM; SYNAPTOSOME; GLUTAMATE; DANTROLENE; CALCIUM CHANNEL BLOCKER;

D O I：

10.1016/0006-8993(95)00185-S

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effects of human immunodeficiency virus type-1 (HIV-1) coat protein gp120 on levels of intrasynaptosomal calcium ([Ca2+](i)) were determined in rat cortical synaptosomes. gp120 at concentrations of greater than or equal to 400 pM, significantly (P < 0.05) increased levels of [Ca2+](i). Treatment with 20 mM KCl, reduced the concentrations of gp120 necessary to produce significant (P < 0.001) increases in [Ca2+](i). gp120-evoked increases in [Ca2+](i) were prevented either by treatment with dantrolene or by removal of extracellular calcium with BAPTA. The peak levels of gp120-induced increases in [Ca2+](i) were not affected by calcium channel blockers lanthanum and nicardipine, by glutamate receptor antagonists MK-801 and NBQX, or by removal of endogenous glutamate with glutamate dehydrogenase. gp120-induced [Ca2+](i) increases in presynaptic terminals may play a role in HIV-mediated effects in the central nervous system.

引用

页码：200 / 206

页数：7

共 38 条

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