IMPURITY PROFILING OF CLARITHROMYCIN USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

被引：13

作者：

MORGAN, D

CUGIER, P

MARELLO, B

SAROCKA, C

STROZ, D

PLASZ, A

机构：

[1] Analytical Research Department, Abbott Laboratories, Department 417 R1B, North Chicago

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 502卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(01)89599-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Clarithromycin is a semi-synthetic antibiotic. High-performance liquid chromatography is used to identify and estimate both manufacturing and degradation impurities. The sample is chromatographed on a YMCC18 column using an eluent of acetonitrile-0.033 M KH2PO4 (48:52) at an apparent pH of 5.4 and ultraviolet detection at 205 nm. Due to the limited availability of impurities, all related substances are referenced to a single impurity standard. A computing integrator is programmed to compensate for differences in detector responses and to identify and calculate the % (w/w) of all known impurities. The majority of all currently identified impurities are detectable at the 0.10% (w/w) level. © 1990.

引用

页码：351 / 358

页数：8

共 8 条

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