METHOD FOR MICROSCALE ISOLATION OF SULFATED GLYCOPEPTIDE FROM TISSUE

被引：8

作者：

ENDO, M ^{[1
]}

YOSIZAWA, Z ^{[1
]}

机构：

[1] TOHOKU UNIV, SCH MED, DEPT BIOCHEM, SENDAI, MIYAGI 980, JAPAN

来源：

TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE | 1979年 / 129卷 / 03期

关键词：

D O I：

10.1620/tjem.129.225

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Endo, M. and Yosizawa, Z. A Method for Micro-Scale Isolation of Sulfated Glycopeptide from Tissue. Tohoku J. exp. Med., 1979, 129 (3), p.225-231 Micro-scale isolation of sulfated glycopeptide from tissue was achieved by successive application of pronase digestion (Step 1), cetylpyridinium chloride-fractionation (Step 2), crude heparinase digestion or chondroitinase ABC digestion plus nitrous acid treatment (Step 3) and preparative cellulose acetate membrane-electrophoresis (Step 4). By this method, sulfated glycopeptide was obtained in a high yield from estrogen-treated rabbit uterus. sulfated glycopeptide; rabbit uterus. © 1979, Tohoku University Medical Press. All rights reserved.

引用

页码：225 / 231

页数：7

共 14 条

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