CHARACTERIZATION OF CALCITONIN GENE-RELATED PEPTIDE RECEPTORS AND ADENYLATE-CYCLASE RESPONSE IN THE MURINE MACROPHAGE CELL-LINE P388-D1

被引:40
作者
ABELLO, J [1 ]
KAISERLIAN, D [1 ]
CUBER, JC [1 ]
REVILLARD, JP [1 ]
CHAYVIALLE, JA [1 ]
机构
[1] HOP EDOUARD HERRIOT,CNRS,URA 1177,INSERM,U80,F-69437 LYONS 3,FRANCE
关键词
D O I
10.1016/0143-4179(91)90072-Q
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Specific receptors for calcitonin gene-related peptide (CGRP) were identified and characterized on plasma membranes from the interleukin-1 secreting murine macrophage-like cells line P388 D1. The binding of [I-125]-rat CGRP I was time-dependent, reversible and the rate of dissociation of [I-125]-rat CGRP I increased in the presence of GTP. Scatchard analysis was consistent with a single class of binding sites, with an apparent dissociation constant of 1.76 nM and a maximal binding capacity of 85.48 fmol/mg protein. In competitive displacement studies, rat CGRP I, human CGRP I and human CGRP II were equipotent to inhibit the binding of [I-125]-rat CGRP I (IC50 = 4nM) while rat CGRP II and the synthetic analogue [tyr-degrees]-human CGRP I were ten-fold less potent. Porcine calcitonin and VIP did not inhibit tracer binding. In the presence of GTP, CGRP stimulation of adenylate cyclase was dose-dependent and strongly correlated with receptor occupation. These results indicate that the P388 D1 macrophage-like cell line expresses CGRP specific receptors functionally coupled to adenylate cyclase, which may be involved in CGRP-mediated macrophage immune response.
引用
收藏
页码:43 / 49
页数:7
相关论文
共 24 条
  • [1] ALTERNATIVE RNA PROCESSING IN CALCITONIN GENE-EXPRESSION GENERATES MESSENGER-RNAS ENCODING DIFFERENT POLYPEPTIDE PRODUCTS
    AMARA, SG
    JONAS, V
    ROSENFELD, MG
    ONG, ES
    EVANS, RM
    [J]. NATURE, 1982, 298 (5871) : 240 - 244
  • [2] NEUROTENSIN - MACROPHAGE INTERACTION - SPECIFIC BINDING AND AUGMENTATION OF PHAGOCYTOSIS
    BARSHAVIT, Z
    TERRY, S
    BLUMBERG, S
    GOLDMAN, R
    [J]. NEUROPEPTIDES, 1982, 2 (06) : 325 - 335
  • [3] CALCITONIN GENE-RELATED PEPTIDE-I AND PEPTIDE-II AND CALCITONIN - DISTINCT EFFECTS ON GASTRIC-ACID SECRETION IN HUMANS
    BEGLINGER, C
    BORN, W
    HILDEBRAND, P
    ENSINCK, JW
    BURKHARDT, F
    FISCHER, JA
    GYR, K
    [J]. GASTROENTEROLOGY, 1988, 95 (04) : 958 - 965
  • [4] NEURO-ENDOCRINE REGULATION OF MUCOSAL IMMUNITY
    BIENENSTOCK, J
    CROITORU, K
    ERNST, PB
    STEAD, RH
    STANISZ, A
    [J]. IMMUNOLOGICAL INVESTIGATIONS, 1989, 18 (1-4) : 69 - 76
  • [5] CALCITONIN GENE-RELATED PEPTIDE IS A POTENT VASODILATOR
    BRAIN, SD
    WILLIAMS, TJ
    TIPPINS, JR
    MORRIS, HR
    MACINTYRE, I
    [J]. NATURE, 1985, 313 (5997) : 54 - 56
  • [6] INTERACTION OF CALCITONIN AND CALCITONIN GENE RELATED PEPTIDE AT RECEPTOR-SITES IN TARGET TISSUES
    GOLTZMAN, D
    MITCHELL, J
    [J]. SCIENCE, 1985, 227 (4692) : 1343 - 1345
  • [7] HARTUNG HP, 1986, J IMMUNOL, V136, P3856
  • [8] LOWRY OH, 1951, J BIOL CHEM, V193, P265
  • [9] AUTORADIOGRAPHIC LOCALIZATION OF CALCITONIN GENE-RELATED PEPTIDE (CGRP) BINDING-SITES IN HUMAN AND GUINEA-PIG LUNG
    MAK, JCW
    BARNES, PJ
    [J]. PEPTIDES, 1988, 9 (05) : 957 - 963
  • [10] MULDERRY PK, 1988, NEUROSCIENCE, V25, P195