ECDYSONE 3-EPIMERASE FROM THE MIDGUT OF MANDUCA-SEXTA (L)

被引：26

作者：

MAYER, RT ^{[1
]}

DURRANT, JL ^{[1
]}

HOLMAN, GM ^{[1
]}

WEIRICH, GF ^{[1
]}

SVOBODA, JA ^{[1
]}

机构：

[1] USDA SEA, BELTSVILLE AGR RES CTR, INSECT PHYSIOL LAB, BELTSVILLE, MD 20705 USA

来源：

STEROIDS | 1979年 / 34卷 / 05期

关键词：

D O I：

10.1016/S0039-128X(79)80016-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ecdysone 3-epimerase was partially purified by ammonium sulfate fractionation from the 100,000 g supernate of M. sexta midguts. The enzyme converts ecdysone and 20-hydroxyecdysone to their respective 3-epimers, requires NADH or NADPH and O2 for this reaction and has the following kinetic parameters: for ecdysone, Km = 17.0 .+-. 1.4 .mu.M, Vmax = 110.6 .+-. 14.6 pmol min-1 mg-1; for 20-hydroxyecdysone, Km = 47.3 .+-. 7.5 .mu.M, Vmax = 131.0 .+-. 3.5 pmol min-1 mg-1; for NADPH, Km = 85.4 .+-. 10.6 .mu.M; for NADH, Km = 51.3 .+-. 1.3 .mu.M. The reaction is irreversible and can be inhibited by various ecdysteroids.

引用

页码：555 / 562

页数：8

共 17 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2]

GILBERT LI, 1973, PHYSL INSECTA, V1

[3]

HOLMAN GM, 1978, INSECT BIOCHEM, V8, P275

[4] METABOLISM OF 4-(C-14)22,25-BISDEOXYECDYSONE DURING LARVAL DEVELOPMENT IN TOBACCO HORNWORM, MANDUCA-SEXTA (L) [J].

KAPLANIS, JN ;

THOMPSON, MJ ;

LINDQUIS.FL ;

ROBBINS, WE ;

DUTKY, SR .

STEROIDS, 1972, 20 (01) :105-&

[5] 3-DEHYDROECDYSONE, A METABOLITE OF ECDYSONE IN CALLIPHORA ERYTHROCEPHALA MEIGEN [J].

KARLSON, P ;

BUGANY, H ;

DOPP, H ;

HOYER, GA .

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1972, 353 (10) :1610-1614

[6] ECDYSONE OXIDASE - REACTION AND SPECIFICITY [J].

KOOLMAN, J ;

KARLSON, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 89 (02) :453-460

[7] ENZYMATIC AND CHEMICAL SYNTHESIS OF 3-DEHYDROECDYSTERONE, A METABOLITE OF MOLTING HORMONE OF INSECTS [J].

KOOLMAN, J ;

SPINDLER, KD .

HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1977, 358 (10) :1339-1344

[8] THIN-LAYER CHROMATOGRAPHIC INSITU ANALYSIS OF INSECT ECDYSONES VIA FLUORESCENCE-QUENCHING [J].

MAYER, RT ;

SVOBODA, JA .

STEROIDS, 1978, 31 (01) :139-150

[9]

MAYER RT, 1970, HOPPESEYLERS Z PHYSL, V359, P1247

[10] ECDYSONE METABOLISM - ECDYSONE DEHYDROGENASE-ISOMERASE [J].

NIGG, HN ;

SVOBODA, JA ;

THOMPSON, MJ ;

KAPLANIS, JN ;

DUTKY, SR ;

ROBBINS, WE .

LIPIDS, 1974, 9 (12) :971-974

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