IMPROVEMENT OF THE TISSUE-CULTURE RESPONSE OF SEED-DERIVED CALLUS-CULTURES OF POA-PRATENSIS L - EFFECT OF GELLING AGENT AND ABSCISIC-ACID

被引：25

作者：

VANARK, HF ^{[1
]}

ZAAL, MACM ^{[1
]}

CREEMERSMOLENAAR, J ^{[1
]}

VANDERVALK, P ^{[1
]}

机构：

[1] CTR PLANT BREEDING & REPROD RES, DLO, DEPT CELL BIOL, 6700 AA WAGENINGEN, NETHERLANDS

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1991年 / 27卷 / 03期

关键词：

SOMATIC EMBRYOGENESIS;

D O I：

10.1007/BF00157591

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of gelling agent and abscisic acid on the morphogenetic response of seed-derived callus cultures of Poa pratensis L. were investigated. On medium solidified with Gelrite, the regeneration frequency of the calluses was twice as high as compared to agar-solidified medium. The average number of green shoots per regenerating callus was not influenced by the type of gelling agent used. When abscisic acid was added to the differentiation medium only, or to both the differentiation medium and the regeneration medium, the percentage of calluses with somatic embryos or embryo-like structures increased (up to 29.6%) as compared to the control (16.4%). The plant formation frequency, however, was not affected by abscisic acid.

引用

页码：275 / 280

页数：6

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