ENZYME-RELEASE KINETICS IN A CELL DISRUPTION CHAMBER OF A BEAD MILL

被引：22

作者：

MELENDRES, AV ^{[1
]}

HONDA, H ^{[1
]}

SHIRAGAMI, N ^{[1
]}

UNNO, H ^{[1
]}

机构：

[1] TOKYO INST TECHNOL,DEPT BIOENGN,4259 NAGATSUTA CHO,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN

来源：

JOURNAL OF CHEMICAL ENGINEERING OF JAPAN | 1993年 / 26卷 / 02期

关键词：

BIOCHEMICAL ENGINEERING; CELL DISRUPTION; ENZYME RELEASE; 2-STEP PROCESS; BEAD MILL; CELL WALL; CYTOPLASM; MEMBRANE-BOUND ENZYME;

D O I：

10.1252/jcej.26.148

中图分类号：

TQ [化学工业];

学科分类号：

0817 ;

摘要：

The kinetics of enzyme release from yeast using a bead mill was investigated. The enzymes investigated were invertase, acid phosphatase, alcohol dehydrogenase and alkaline phosphatase. A two-step consecutive process model was proposed to describe the behavior of enzyme release. The model described well the release of both membrane-bound and non membrane-bound enzymes, such as alkaline phosphatase and other enzymes, respectively.

引用

页码：148 / 152

页数：5

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