LIPOXIN A4 INDUCES NEUTROPHIL-DEPENDENT CYTOTOXICITY FOR HUMAN ENDOTHELIAL-CELLS

被引:15
作者
BRATT, J
LERNER, R
RINGERTZ, B
PALMBLAD, J
机构
[1] KAROLINSKA INST,DEPT RHEUMATOL,STOCKHOLM,SWEDEN
[2] KAROLINSKA INST,DEPT MED,STOCKHOLM,SWEDEN
关键词
D O I
10.1111/j.1365-3083.1994.tb03385.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The authors have assessed the capacity of neutrophil granulocytes (PMN) to kill cultured human umbilical-vein endothelial cells (HUVEC) in vitro (as release of Cr-51) in response to the recently described double dioxygenation product of arachidonic acid, lipoxin A(4) (LXA4). LXA4 conferred a marked cytotoxicity, whereas formyl-methionyl-leucyl-phenylalanine (fMLP) was less potent. The LXA4 and fMLP effects were dose dependent, with a maximum at 100 nM (which caused 2.7- and 2.3-fold increases of Cr-51 release, respectively, relative to buffer-treated controls). The LXA4 and fMLP responses increased with the PMN concentration, depended on the fetal calf serum concentration, incubation temperature and duration and the presence of calcium and magnesium ions.
引用
收藏
页码:351 / 354
页数:4
相关论文
共 23 条