BA SIMPLE AND RAPID METHOD FOR DETERMINATION OF FLUCONAZOLE IN HUMAN PLASMA SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：12

作者：

FLORESMURRIETA, FJ

GRANADOSSOTO, V

HONG, E

机构：

[1] Seccion de Terapéutica Experimental, Departamento de Farmacología y Toxicología, Centro de Investigacíon y de Estudios Avanzados, del Instituto Politécnico Nacional, Apartado Postal 22026, 14000 Mexico, D.F.

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 17期

关键词：

D O I：

10.1080/10826079408013995

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and simple method for determination of fluconazole in plasma samples by high performance liquid chromatography was developed. The method includes a single extraction of alkalinized plasma with ethyl acetate. Plasma extracts were analyzed on a reverse-phase column eluted with a mixture of acetonitrile and 0.05 M sodium monohydrogen phosphate (pH 4) and detected by absorbance at 210 nm. Retention times for fluconazole and the internal standard were 7 and 13 min respectively. The method was linear in the range of 0.1 to 5 mu g/ml and the detection limit of the method was 20 ng/ml. This method is suitable for determination of fluconazole after administration of the drug at therapeutic doses, and it could be used in pharmacokinetic studies of the drug in humans.

引用

页码：3803 / 3811

页数：9

共 6 条

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