DUAL EFFECT OF DEFEROXAMINE ON FREE-RADICAL FORMATION AND REOXYGENATION INJURY IN ISOLATED HEPATOCYTES

被引:18
作者
CARACENI, P
VANTHIEL, DH
BORLE, AB
机构
[1] UNIV PITTSBURGH, SCH MED, DEPT PHYSIOL, PITTSBURGH, PA 15261 USA
[2] UNIV PITTSBURGH, SCH MED, DEPT SURG, PITTSBURGH, PA 15261 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | 1995年 / 269卷 / 01期
关键词
SUPEROXIDE FORMATION; HYDROXYL RADICAL FORMATION; LUCIGENIN CHEMILUMINESCENCE; LIPID PEROXIDATION; IRON CHELATION; FREE RADICAL SCAVENGING;
D O I
10.1152/ajpgi.1995.269.1.G132
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The effects of low concentrations (10 and 100 mu M) and high concentrations (1, 10, and 20 mM) of deferoxamine (DFO) on superoxide (O-2-radical anion) formation, lipid peroxidation, and cell injury were studied in freshly isolated perfused rat hepatocytes during a 2-h reoxygenation period after 2.5 h of anoxia. O-2-radical-anion production was measured by lucigenin-enhanced chemiluminescence, lipid peroxidation by malondialdehyde (MDA) formation, and cell injury by lactate dehydrogenase (LDH) release. On reoxygenation and in the absence of DFO, O-2-radical-anion generation increased 11-fold, MDA increased 3.7-fold, and LDH release practically doubled. Low concentrations of DFO had no effect on O-2-radical-anion generation but decreased MDA and LDH release from 44 to 75%. High concentrations of DFO significantly depressed O-2-radical-anion formation, with very little additional effect on MDA or LDH release. These experiments illustrate in a biological system the dual effect of DFO: 1) at low concentrations, DFO acts as a specific iron chelator and inhibits lipid peroxidation and cell. injury without preventing O-2-radical-anion formation, and 2) at high concentrations, DFO acts as a nonspecific scavenger of oxygen free radicals such as O-2-radical-anion.
引用
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页码:G132 / G137
页数:6
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