ISOLATION AND CHARACTERIZATION OF A GLYCOSPHINGOLIPID HAVING A NEW SIALIC ACID

A glycosphingolipid, having a carbohydrate structure similar to that of hematoside but with a higher migration rate on thin-layer chromatography, was isolated from equine erythrocyte membranes. The sialosyl residue of this glycolipid was not hydrolyzed by neuraminidase. The sialic acid, isolated from the acid hydrolysate, was identified as a new sialic acid, O-acetyl((N-glycolyl)neuraminic acid. After removal of O-acetyl group, the sialic acid of this glycolipid was hydrolyzable by neuraminidase and its migration rate on thinlayer chromatography was similar to that of known hematoside. The neuraminidase resistant property of the O-acetyl-(N-glycolyl)neuraminosyl was discussed. © 1969, American Chemical Society. All rights reserved.