A RAPID INSITU EXPRESSION TEST FOR VIRULENCE-ASSOCIATED PROTEINS OF YERSINIA-ENTEROCOLITICA

被引：5

作者：

NAUMANN, M

HANSKI, C

FRIEDRICH, B

RIECKEN, EO

机构：

[1] FREE UNIV BERLIN,KLINIKUM STEGLITZ,INNERE MED SCHWERPUNKT GASTROENTEROL,HINDENBURGDAMM 30,W-1000 BERLIN 45,GERMANY

[2] FREE UNIV BERLIN,INST PFLANZENPHYSIOL ZELLBIOL & MIKROBIOL,W-1000 BERLIN 33,GERMANY

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1990年 / 11卷 / 02期

关键词：

Expression test; Virulence-associated protein; Yersinia enterolitica;

D O I：

10.1016/0167-7012(90)90008-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure is presented for screening Yersinia enterolitica strains expressing plasmid-coded outer membrane proteins. Colonies were transfered from agar plates onto nitrocellulose filters and bacterial proteins were fixed with formaldehyde. The plasmid-bearing bacteria were detected by monospecific rabbit antisera against virulence-associated proteins, followed by gold-labeled anti-rabbit immunoglobulins and silver enhancement. Polyspecific and monospecific antisera revealed in situ high sensitivity for virulence proteins and allowed screening also at high colony density. The application of the procedure for screening a Yersinia plasmid DNA library resulted in detection of clones expressing virulence proteins. © 1990.

引用

页码：83 / 93

页数：11

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