EVIDENCE THAT RAT-LIVER PYRUVATE-DEHYDROGENASE KINASE ACTIVATOR PROTEIN IS A PYRUVATE-DEHYDROGENASE KINASE

被引：19

作者：

MISTRY, SC ^{[1
]}

PRIESTMAN, DA ^{[1
]}

KERBEY, AL ^{[1
]}

RANDLE, PJ ^{[1
]}

机构：

[1] JOHN RADCLIFFE HOSP,NUFFIELD DEPT CLIN BIOCHEM,OXFORD OX3 9DU,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1991年 / 275卷

关键词：

D O I：

10.1042/bj2750775

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is shown here that rat liver pyruvate dehydrogenase (PDH) kinase activator protein (KAP) catalyses ATP-dependent inactivation and [P-32]phosphorylation of pig heart PDHE1 and of yeast (Saccharomyces cerevisiae) PDH complex devoid of PDH kinase activity, that fluorosulphonylbenzoyladenosine inactivates rat liver KAP and the intrinsic PDH kinase of rat liver PDH complex, and that KAP, like PDH kinase, is inactivated by thiol-reactive reagents. It is concluded that KAP is a free PDH kinase.

引用

页码：775 / 779

页数：5

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