UREA SENSORS BASED ON GLASS PH ELECTRODES WITH PHYSICALLY IMMOBILIZED UREASE

被引：45

作者：

KONCKI, R

LESZCZYNSKI, P

HULANICKI, A

GLAB, S

机构：

[1] Department of Chemistry, Warsaw University, 02-093 Warsaw

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 257卷 / 01期

关键词：

POTENTIOMETRY; BIOSENSOR; PH; UREA;

D O I：

10.1016/0003-2670(92)80151-V

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Different gels were used as a matrix for physical enzyme immobilization on the sensing surface of glass pH electrodes. The recommended technique of immobilization is based on enzyme entrapment within a gel prepared in situ on the glass electrode surface. Poly(vinyl chloride), cellulose trinitrate and especially cellulose triacetate are the best matrix materials of urease immobilization. The effect of buffer pH and buffer capacity and the effect of the stirring rate were investigated and the optimum measurement conditions are discussed.

引用

页码：67 / 72

页数：6

共 21 条

[1] A COATED-METAL ENZYME ELECTRODE FOR UREA DETERMINATIONS [J].