GENOME STRUCTURE OF A VIRUS INFECTING THE MARINE BROWN ALGA ECTOCARPUS-SILICULOSUS

被引：46

作者：

LANKA, STJ ^{[1
]}

KLEIN, M ^{[1
]}

RAMSPERGER, U ^{[1
]}

MULLER, DG ^{[1
]}

KNIPPERS, R ^{[1
]}

机构：

[1] UNIV CONSTANCE,DIV BIOL,W-7750 CONSTANCE,GERMANY

来源：

VIROLOGY | 1993年 / 193卷 / 02期

关键词：

D O I：

10.1006/viro.1993.1189

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We describe a procedure for the isolation of virus particles from the marine brown alga Ectocarpus siliculosus. Virus particles are composed of at least 13 different polypeptides, including two glycoproteins, and double-stranded DNA. A typical virus DNA preparation contains three fractions, namely linear DNA and circular DNA, each composed of about 320 kilobase pairs, as well as DNA fragments, 10 to 60 kilobase pairs in size. The large linear and the circular DNA contain single-stranded regions (average length: 2.9 kilobases). We propose that the native Ectocarpus virus genome is a circular DNA molecule whose double strand is interrupted by single-stranded regions. During the preparation procedure, the DNA circles tend to break at the single-stranded sites producing large linear as well as fragmented DNA. © 1993 Academic Press. All rights reserved.

引用

页码：802 / 811

页数：10

共 29 条

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