A NEW ACRYLIC RESIN FORMULATION - A USEFUL TOOL FOR HISTOLOGICAL, ULTRASTRUCTURAL, AND IMMUNOCYTOCHEMICAL INVESTIGATIONS

被引:203
作者
SCALA, C
CENACCHI, G
FERRARI, C
PASQUINELLI, G
PREDA, P
MANARA, GC
机构
[1] UNIV PARMA,CTR MICROSCOPIE ELECTR,I-43100 PARMA,ITALY
[2] UNIV PARMA,IST CLIN DERMATOL,I-43100 PARMA,ITALY
关键词
ACRYLIC HYDROPHILIC RESIN; AROMATIC CROSS-LINKER; HISTOLOGICAL STAININGS; IMMUNOHISTOCHEMISTRY; ULTRASTRUCTURE; IMMUNOELECTRON MICROSCOPY;
D O I
10.1177/40.11.1431065
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We describe a new formulation for a hydrophilic resin, mostly composed of glycol methacrylate and hydroxypropyl methacrylate and here referred to as bioacryl, that allows the performance of morphological and immunohistochemical investigations at both light and electron microscopic levels. Immunolocalizations performed on bioacryl-embedded tissues are characterized by high specificity with virtually absent background staining. Finally, the new resin yields satisfactory fine-structural preservation, resulting in ultrastructural images of better quality than those obtained with Lowicryl K4M.
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页码:1799 / 1804
页数:6
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