RIBOZYMES DESIGNED TO INHIBIT TRANSFORMATION OF NIH3T3 CELLS BY THE ACTIVATED C-HA-RAS GENE

被引：71

作者：

KOIZUMI, M ^{[1
]}

KAMIYA, H ^{[1
]}

OHTSUKA, E ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

GENE | 1992年 / 117卷 / 02期

关键词：

HAMMERHEAD RIBOZYME; SELF-CLEAVING RNA; ANTISENSE RNA; GENE REGULATION;

D O I：

10.1016/0378-1119(92)90727-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have designed hammerhead ribozymes that cleave c-Ha-ras mRNA mutated at codon 12 (GGU --> GUU). Plasmids containing the ribozyme-encoding genes were expressed under the control of the long terminal repeats of Rous sarcoma virus in NIH3T3 cells transfected with the activated c-Ha-ras gene. These ribozymes were found to inhibit formation of foci (by about 50%) by cleaving the oncogene mRNA, rather than by hybridizing to it. Furthermore, when the activated c-Ha-ras gene was cotransfected with the ribozyme-encoding gene, three morphologically flat colonies were found and isolated. We also found that expression of c-Ha-ras was suppressed in cells containing ribozymes.

引用

页码：179 / 184

页数：6

共 24 条

[1] CELLULAR MUTATION MEDIATES T-ANTIGEN-POSITIVE REVERTANT CELLS RESISTANT TO SIMIAN VIRUS-40 TRANSFORMATION BUT NOT TO RETRANSFORMATION BY POLYOMAVIRUS AND ADENOVIRUS TYPE-2 [J].

BAUER, M ;

GUHL, E ;

GRAESSMANN, M ;

GRAESSMANN, A .

JOURNAL OF VIROLOGY, 1987, 61 (06) :1821-1827

[2] NONENZYMATIC CLEAVAGE AND LIGATION OF RNAS COMPLEMENTARY TO A PLANT-VIRUS SATELLITE RNA [J].

BUZAYAN, JM ;

GERLACH, WL ;

BRUENING, G .

NATURE, 1986, 323 (6086) :349-353

[3] SPECIFIC GENE SUPPRESSION BY ENGINEERED RIBOZYMES IN MONKEY CELLS [J].

CAMERON, FH ;

JENNINGS, PA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (23) :9139-9143

[4] RIBOZYME MEDIATED DESTRUCTION OF RNA INVIVO [J].

COTTEN, M ;

BIRNSTIEL, ML .

EMBO JOURNAL, 1989, 8 (12) :3861-3866

[5] SELF-CLEAVING TRANSCRIPTS OF SATELLITE DNA FROM THE NEWT [J].

EPSTEIN, LM ;

GALL, JG .

CELL, 1987, 48 (03) :535-543

[6] SELF-CLEAVAGE OF PLUS AND MINUS RNAS OF A VIRUSOID AND A STRUCTURAL MODEL FOR THE ACTIVE-SITES [J].

FORSTER, AC ;

SYMONS, RH .

CELL, 1987, 49 (02) :211-220

[7] HIGH-EFFICIENCY DNA-MEDIATED TRANSFORMATION OF PRIMATE CELLS [J].

GORMAN, C ;

PADMANABHAN, R ;

HOWARD, BH .

SCIENCE, 1983, 221 (4610) :551-553

[8] NEW TECHNIQUE FOR ASSAY OF INFECTIVITY OF HUMAN ADENOVIRUS 5 DNA [J].

GRAHAM, FL ;

VANDEREB, AJ .

VIROLOGY, 1973, 52 (02) :456-467

[9] SIMPLE RNA ENZYMES WITH NEW AND HIGHLY SPECIFIC ENDORIBONUCLEASE ACTIVITIES [J].

HASELOFF, J ;

GERLACH, WL .

NATURE, 1988, 334 (6183) :585-591

[10] SPECIFIC REGULATION OF GENE-EXPRESSION BY ANTISENSE, SENSE AND ANTIGENE NUCLEIC-ACIDS [J].

HELENE, C ;

TOULME, JJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1049 (02) :99-125

← 1 2 3 →