TRANSFORMATION OF SALMONELLA-TYPHIMURIUM TA1538 BY OPTIMIZED ELECTROPORATION

被引：2

作者：

GENTIL, M ^{[1
]}

AZAS, N ^{[1
]}

DEMEO, M ^{[1
]}

LAGET, M ^{[1
]}

DUMENIL, G ^{[1
]}

机构：

[1] FAC PHARM MARSEILLE,MICROBIOL LAB,27 BD JEAN MOULIN,F-13385 MARSEILLE 5,FRANCE

来源：

BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 04期

关键词：

D O I：

10.1007/BF00150894

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Standard electroporation of Salmonella typhimurium TA1538 by the plasmid pAMH70 yielded 1.3x10(2) transformants/mug of plasmid DNA. Three parameters: resistance (U1), voltage (U2) and dose of plasmid DNA(U3) Were optimized with two Doehlert designs. Transformation was increased 75 fold. Optimal values for U1, U2 and U3 were 761.0 OMEGA, 1.6 kV and 96.1 ng respectively. The most important parameters were U2 and the couple U1/U2.

引用

页码：255 / 260

页数：6

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