AN EFFICIENT SHINE-DALGARNO SEQUENCE BUT NOT TRANSLATION IS NECESSARY FOR LACZ MESSENGER-RNA STABILITY IN ESCHERICHIA-COLI

被引：49

作者：

WAGNER, LA

GESTELAND, RF

DAYHUFF, TJ

WEISS, RB

机构：

[1] UNIV UTAH, DEPT HUMAN GENET, SALT LAKE CITY, UT 84132 USA

[2] UNIV UTAH, HOWARD HUGHES MED INST, SALT LAKE CITY, UT 84132 USA

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 06期

关键词：

D O I：

10.1128/jb.176.6.1683-1688.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The 5' ends of many bacterial transcripts are important in determining mRNA stability. A series of Shine-Dalgarno (SD) sequence changes showed that the complementarity of the SD sequence to the anti-SD sequence of 16S rRNA correlates with lacZ mRNA stability in Escherichia coli. Several initiation codon changes showed that an efficient initiation codon is not necessary to maintain lacZ mRNA stability. A stop codon in the 10th codon of lacZ increased mRNA stability. Therefore, ribosomal binding via the SD sequence but not translation of the coding region is necessary to maintain lacZ mRNA stability.

引用

页码：1683 / 1688

页数：6

共 39 条

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