SYNTHESIS OF ASPARTAME PRECURSOR WITH AN IMMOBILIZED THERMOLYSIN IN MIXED ORGANIC-SOLVENTS

被引：21

作者：

MIYANAGA, M ^{[1
]}

TANAKA, T ^{[1
]}

SAKIYAMA, T ^{[1
]}

NAKANISHI, K ^{[1
]}

机构：

[1] OKAYAMA UNIV,FAC ENGN,DEPT BIOTECHNOL,OKAYAMA 700,JAPAN

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1995年 / 46卷 / 06期

关键词：

ENZYMATIC SYNTHESIS; THERMOLYSIN; IMMOBILIZED ENZYME; ASPARTAME PRECURSOR; ORGANIC SOLVENT;

D O I：

10.1002/bit.260460617

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

N-(benzyloxycarbonyl)-L-aspartyl-L-phenylalanine methyl ester, a precursor of the synthetic sweetener, aspartame, was synthesized from N-(benzyloxycarbonyl])-L-aspartic acid and L-phenylalanine methyl ester with an immobilized thermolysin (EC 3.4.24.4) in the mixed organic solvent system of tert-amyl alcohol and ethyl acetate. A mixed solvent consisting of tert-amyl alcohol and ethyl acetate at a ratio of 33:67 (v/v) was found to be the most suitable with respect to synthetic rate and stability of the immobilized enzyme. The reaction continued to proceed quite successfully in a column reactor at 40 degrees C and at a space velocity of 3.6 h(-1) with a yield of 99%, using 40 mM Z-Asp and 200 mM PheOMe dissolved in the mixed solvent as the substrate. (C) 1995 John Wiley & Sons, Inc.

引用

页码：631 / 635

页数：5

共 7 条

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[5] LONG-TERM CONTINUOUS SYNTHESIS OF ASPARTAME PRECURSOR IN A COLUMN REACTOR WITH AN IMMOBILIZED THERMOLYSIN [J].

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