SIMULTANEOUS VISUALIZATION OF Q-BANDS AND FISH SIGNALS USING A NOVEL FLUOROCHROME

被引：12

作者：

KONDOH, Y

ONO, T

KAGIYAMA, N

FUJITA, S

MOMIYAMA, M

HORI, SH

YOSHIDA, MC

机构：

[1] HOKKAIDO UNIV,CHROMOSOME RES UNIT,SAPPORO,HOKKAIDO 060,JAPAN

[2] HOKKAIDO UNIV,DEPT ZOOL,SAPPORO,HOKKAIDO 060,JAPAN

[3] HOKKAIDO UNIV,FAC SCI,GRAD SCH ENVIRONM EARTH SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

CYTOGENETICS AND CELL GENETICS | 1995年 / 71卷 / 01期

关键词：

D O I：

10.1159/000134071

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We developed a new HNPP-azo dye method for detection of fluorescence in situ hybridization (FISH) signals on Q-banded chromosomes by use of a newly synthesized fluorochrome, HNPP (3-hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester), which reacts enzymatically with alkaline phosphatase and azo dye. The FISH staining method permits simultaneous detection of orange HNPP signals on chromosomal sites labeled by Q-banding, allowing the assignment of small (440-1,200 bp) probes.

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页码：96 / 98

页数：3

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