AFFINITY IMMUNOBLOTTING DETECTION OF SERUM MONOCLONAL IMMUNOGLOBULINS REACTIVE WITH GLYCOSPHINGOLIPIDS

被引：10

作者：

JAUBERTEAU, MO

COOK, JM

DROUET, M

PREUDHOMME, JL

机构：

[1] UNIV HOSP LIMOGES,IMMUNOL LAB,F-87042 LIMOGES,FRANCE

[2] UNIV HOSP LIMOGES,INSERM,CJF 8803,F-87042 LIMOGES,FRANCE

[3] UNIV HOSP POITIERS,IMMUNOL & IMMUNOPATHOL LAB,CNRS,URA 1172,F-86021 POITIERS,FRANCE

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 134卷 / 01期

关键词：

Affinity filter; Glycosphingolipid; Immunoblot; Monoclonal immunoglobulin; Neuropathy;

D O I：

10.1016/0022-1759(90)90118-F

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The characterization of serum monoclonal IgM reactive with glycosphingolipids present in the nervous system is of major importance in patients with certain neuropathies. A rapid, sensitive and specific one-step method which permits such as characterization is described. Serum immunoglobulins separated by high resolution agarose electrophoresis are transferred to affinity filters (nitrocellulose sheets coated with glycolipids), and revealed with enzyme-tagged monospecific anti-immunoglobulin antibodies. © 1990.

引用

页码：107 / 112

页数：6

共 27 条

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AUCOUTURIER P, 1987, REV I PASTEUR LYON, V20, P147

[2] MYELIN-ASSOCIATED GLYCOPROTEIN IS THE ANTIGEN FOR A MONOCLONAL IGM IN POLYNEUROPATHY [J].