ISOLATION AND CHARACTERIZATION OF A HUMAN CDNA CLONE ENCODING A NOVEL DNA TOPOISOMERASE-II HOMOLOG FROM HELA-CELLS

被引：53

作者：

AUSTIN, CA ^{[1
]}

FISHER, LM ^{[1
]}

机构：

[1] UNIV LONDON ST GEORGES HOSP,SCH MED,DEPT CELLULAR & MOLEC SCI,MOLEC GENET GRP,CRANMER TERR,LONDON SW17 0RE,ENGLAND

来源：

FEBS LETTERS | 1990年 / 266卷 / 1-2期

关键词：

cDNA cloning; DNA topoisomerase II; Human cell line; Isoenzyme; Protein sequence homology;

D O I：

10.1016/0014-5793(90)81520-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and sequenced 3 human DNA topoisomerase II (topo II) partial cDNA clones from a HeLa carcinoma cell cDNA library. Two clones were identical to an internal fragment of HeLa topo II cDNA. The third clone, CAA5, had a different and novel sequence which shared significant nucleotide (62%) and predicted peptide (70%) homologies with a region of the HeLa topo II cDNA. Our results suggest that HeLa cells express at least two homologous forms of DNA topoisomerase II. The new HeLa topo II homologue is discussed in relation to topo II isoenzymes recently described in a Burkitt lymphoma and other cell lines. © 1990.

引用

页码：115 / 117

页数：3

共 11 条

[1] BIOCHEMICAL AND IMMUNOLOGICAL CHARACTERIZATION OF MAMMALIAN DNA TOPOISOMERASE-II [J].