RECONSTITUTION OF A CHLORIDE-TRANSLOCATING ATPASE FROM APLYSIA-CALIFORNICA GUT

被引：24

作者：

GERENCSER, GA

机构：

[1] Department of Physiology, College of Medicine, University of Florida, Gainesville, FL

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1030卷 / 02期

关键词：

RECONSTITUTION; ATPASE; CL--STIMULATED; CHLORIDE TRANSPORT; DIGITONIN;

D O I：

10.1016/0005-2736(90)90307-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Basolateral membranes of Aplysia foregut epithelia contain both a Cl--stimulated ATPase activity and an ATP-dependent Cl- transport. The protein responsible for both of these biochemical activities (Cl- pump) can be solubilized and reconstituted into liposomes with the aid of the detergent digitonin. Proteoliposomal Cl- pump activity was inhibited by vanadate.

引用

页码：301 / 303

页数：3

共 15 条

[1]

AKERA T, 1983, FED PROC, V42, P2984

[2]

COOPERSTEIN SJ, 1951, J BIOL CHEM, V189, P665

[3]

Fiske CH, 1925, J BIOL CHEM, V66, P375

[4]

GERENCSER GA, 1985, BIOCHIM BIOPHYS ACTA, V816, P415, DOI 10.1016/0005-2736(85)90510-3

[5] IS THERE A CL- PUMP [J].

GERENCSER, GA ;

WHITE, JF ;

GRADMANN, D ;

BONTING, SL .

AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 255 (05) :R677-R692

[6]

GERENCSER GA, 1983, J EXP BIOL, V106, P143

[7] ELECTROGENIC ATP-DEPENDENT CL- TRANSPORT BY PLASMA-MEMBRANE VESICLES FROM APLYSIA INTESTINE [J].

GERENCSER, GA .

AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 254 (01) :R127-R133

[8]

GERENCSER GA, 1985, AM J PHYSIOL, V248, pR241, DOI 10.1152/ajpregu.1985.248.2.R241

[9]

GRADMANN D, 1984, CHLORIDE TRANSPORT C, P13

[10]

HILL BS, 1984, CHLORIDE TRANSPORT C, P1

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