A GENETIC-LINKAGE MAP OF PICEA-ABIES KARST, BASED ON RAPD MARKERS, AS A TOOL IN POPULATION-GENETICS

被引：68

作者：

BINELLI, G ^{[1
]}

BUCCI, G ^{[1
]}

机构：

[1] UNIV PARMA,INST ECOL,I-43100 PARMA,ITALY

来源：

THEORETICAL AND APPLIED GENETICS | 1994年 / 88卷 / 3-4期

关键词：

PICEA ABIES; RAPD; POPULATION GENETICS; LINKAGE MAP;

D O I：

10.1007/BF00223633

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Norway spruce (Picea abies Karst.) is a most important species among European forest trees for both economical and ecological reasons. However, this species has suffered from a lack of information on the genetic side due to the scarcity of linkage data. In this study we have used a population of 72 megagametophytes from a single tree in a natural Italian stand to produce a genetic linkage map by means of RAPD markers. Ninety-six random decamers used as primers yielded 185 polymorphic loci showing Mendelian inheritance. Analysis of the segregation by multipoint analysis allowed us to define 17 major linkage groups covering a total distance of 3584 cM, with an average spacing between markers of 22 cM. Possible uses of a genetic linkage map with respect to population ecology and genetics are discussed.

引用

页码：283 / 288

页数：6

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