Electrochemical detection of DNA hybridization based on carbon-nanotubes loaded with CdS tags

被引：117

作者：

Wang, J ^{[1
]}

Liu, GD

Jan, MR

Zhu, QY

机构：

[1] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA

[2] Univ Peshawar, Dept Chem, Peshawar, Pakistan

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2003年 / 5卷 / 12期

基金：

美国国家科学基金会;

关键词：

DNA hybridization; quantum dots; carbon nanotubes; stripping voltammetry; amplification; nanoparticles;

D O I：

10.1016/j.elecom.2003.09.010

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

An effective method for amplifying electrical detection of DNA hybridization based on carbon-nanotubes (CNT) carrying a large number of US particle tracers is described. Such use of CNT amplification platforms is combined with an ultrasensitive stripping-voltammetric detection of the dissolved CdS tags following dual hybridization events of a sandwich assay on a streptavidin modified 96-well microplate. Anchoring of the monolayer-protected quantum dots to the acetone-activated CNT was accomplished via hydrophobic interactions. SEM images indicate that the nanocrystals are attached along the CNT sidewall, with a loading of around 500 particles per CNT. A substantial (similar to500-fold) lowering of the detection limit is obtained compared to conventional single-particle stripping hybridization assays, reflecting the US loading on the CNT carrier. A large excess (250-fold) of non-complimentary oligonucleotides have minimal effect on the response. Such use of CNT as carriers for multiple electrochemical tags offers great promise for ultrasensitive detection of other biorecognition events. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：1000 / 1004

页数：5

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