V-REGION DIRECTED SELECTION IN DIFFERENTIATING B-LYMPHOCYTES

被引:91
作者
HUETZ, F [1 ]
CARLSSON, L [1 ]
TORNBERG, UC [1 ]
HOLMBERG, D [1 ]
机构
[1] INST PASTEUR,UNITE IMMUNOL,F-75724 PARIS 15,FRANCE
关键词
B-CELL DIFFERENTIATION; IMMUNOGLOBULIN VH GENES; V-REGION SELECTION;
D O I
10.1002/j.1460-2075.1993.tb05830.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We here analyse the repertoire of V(H)7183 rearrangements isolated from different stages of B cell differentiation in adult mice. The nucleotide sequence analyses of V(H)7183-D-J(H) rearrangements derived from large pre-B cells (B220+, mu-), small pre-B cells (B220+, mu-) and mature B cells (B220+, mu+) isolated from adult bone marrow revealed a sequential accumulation, among functional rearrangements, of D segments of the FL16 family and a depletion of D segments using the second and the third reading frame (RF). One member (V(H)7183.1) of the V(H)7183 gene family was utilized in 60-80% of the rearrangements of all populations analysed. In neonates the majority of the rearrangements utilizing this gene was found to be functional. In contrast, >96% of the V(H)7183 rearrangements isolated from adult spleen were non-functional. These data provide evidence for cellular selection of V(H) regions acting at different points of the B cell differentiation pathway and at the transition of B cells from the bone marrow to the periphery.
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页码:1819 / 1826
页数:8
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