PHOTOAFFINITY-LABELING OF PKC WITH A PHORBOL DERIVATIVE - IMPORTANCE OF THE 13-ACYL GROUP IN PHORBOL ESTER-PKC INTERACTION

被引：20

作者：

SODEOKA, M ^{[1
]}

UOTSU, K ^{[1
]}

SHIBASAKI, M ^{[1
]}

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,BUNKYO KU,TOKYO 113,JAPAN

来源：

TETRAHEDRON LETTERS | 1995年 / 36卷 / 48期

关键词：

D O I：

10.1016/0040-4039(95)01864-E

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We report the design and synthesis of a novel photoaffinity ligand, phorbol 12-(1-pyrenebutyrate) 13-diazoacetate (PPDA, 6). This photolabile phorbol derivative labeled with tritium was used for photocrosslinking experiments with a sample of PKC partially purified from rat brain, and specific binding of 6 to PKC was observed.

引用

页码：8795 / 8798

页数：4

共 35 条

[1] ARECES LB, 1994, J BIOL CHEM, V269, P19553
[2] BLUMBERG PM, 1988, CANCER RES, V48, P1
[3] STRUCTURAL ANALOGIES BETWEEN PROTEIN KINASE-C ACTIVATORS
BRASSEUR, R
CABIAUX, V
HUART, P
CASTAGNA, M
BAZTAR, S
RUYSSCHAERT, JM
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 127 (03) : 969 - 976
[4] CASTAGNA M, 1982, J BIOL CHEM, V257, P7847
[5] SPECIFIC LABELING OF MOUSE-BRAIN MEMBRANE PHOSPHOLIPIDS WITH [20-H-3 PHORBOL 12-PARA-AZIDOBENZOATE 13-BENZOATE, A PHOTOLABILE PHORBOL ESTER
DELCLOS, KB
YEH, E
BLUMBERG, PM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (10): : 3054 - 3058
[6] CYTOSOLIC-NUCLEAR TUMOR PROMOTER-SPECIFIC BINDING-PROTEIN (CN-TPBP) IN HUMAN PROMYELOCYTIC LEUKEMIA-CELLS HL-60
HASHIMOTO, Y
SHUDO, K
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 166 (03) : 1126 - 1132
[7] Hecker E, 1984, CELLULAR INTERACTION, P3
[8] SOLUTION STRUCTURE OF A CYSTEINE-RICH DOMAIN OF RAT PROTEIN-KINASE-C
HOMMEL, U
ZURINI, M
LUYTEN, M
[J]. NATURE STRUCTURAL BIOLOGY, 1994, 1 (06): : 383 - 387
[9] SOLUTION STRUCTURE OF CYSTEINE-RICH DOMAIN OF PROTEIN-KINASE C-ALPHA
ICHIKAWA, S
HATANAKA, H
TAKEUCHI, Y
OHNO, S
INAGAKI, F
[J]. JOURNAL OF BIOCHEMISTRY, 1995, 117 (03) : 566 - 574
[10] PHOTOLABILE DERIVATIVES OF INDOLE ALKALOID TUMOR PROMOTER TELEOCIDINS - SYNTHESIS, BIOLOGICAL-ACTIVITIES AND PHOTOAFFINITY-LABELING STUDIES
IRIE, K
OKUNO, S
KOIZUMI, F
KOSHIMIZU, K
NISHINO, H
IWASHIMA, A
[J]. TETRAHEDRON, 1993, 49 (47) : 10817 - 10830

← 1 2 3 4 →