TEMPORAL CHANGES OF C-FOS EXPRESSION IN OXYTOCINERGIC MAGNOCELLULAR NEUROENDOCRINE CELLS OF THE RAT HYPOTHALAMUS WITH RESTRAINT STRESS

被引:54
作者
MIYATA, S [1 ]
ITOH, T [1 ]
LIN, SH [1 ]
ISHIYAMA, M [1 ]
NAKASHIMA, T [1 ]
KIYOHARA, T [1 ]
机构
[1] KYOTO INST TECHNOL,DEPT APPL BIOL,SAKYO KU,KYOTO 606,JAPAN
关键词
C-FOS; OXYTOCIN; RESTRAINT STRESS; SUPRAOPTIC NUCLEUS; PARAVENTRICULAR NUCLEUS;
D O I
10.1016/0361-9230(95)00017-8
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The present experiments were undertaken to examine c-fos expression in magnocellular neuroendocrine cells (MNCs) of the rat hypothalamus with restraint stress using dual immunohistochemistry for c-fos and oxytocin. Restraint stress induced c-fos expression in oxytocinergic MNCs in the supraoptic nucleus (SON) and paraventricular nucleus (PVN). Quantitative immunohistochemical analysis revealed that percentages of c-fos-positive cells to oxytocin-immunoreactive MNCs in the SON and PVN maximally increased at 2 h after restraint stress had started, and began to decline in spite of the fact that the restraint of animals were continued. Similar results were obtained from time course of c-fos expression in parvocellular neurons of the PVN. When animals were released to move freely in their home cages following the 3-h restraint, the plasma levels of oxytocin declined to reach basal levels within 30 min and c-fos immunoreactivity in the hypothalamic MNCs and parvocellular neurons disappeared faster than those of the continually restrained, These results demonstrate that restraint stress induces c-fos expression in oxytocinergic MNCs in the SON and PVN, and that time course of c-fos expression is transient even in the continuation of restraint stress.
引用
收藏
页码:391 / 395
页数:5
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