SPECTROPHOTOMETRIC DETERMINATION OF MYOGLOBIN IN CARDIAC AND SKELETAL-MUSCLE - SEPARATION FROM HEMOGLOBIN BY SUBUNIT-EXCHANGE CHROMATOGRAPHY

被引：59

作者：

SCHUDER, S ^{[1
]}

WITTENBERG, JB ^{[1
]}

HASELTINE, B ^{[1
]}

WITTENBERG, BA ^{[1
]}

机构：

[1] YESHIVA UNIV,ALBERT EINSTEIN COLL MED,DEPT PHYSIOL,BRONX,NY 10461

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 92卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90687-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Myoglobin is extracted from muscle and separated from blood hemoglobin by subunit-exchange chromatography on a column of Sepharose 4B to which hemoglobin α-β subunits are linked covalently. Hemoglobin is retained on the column. Myoglobin in the effluent is determined spectrophotometrically as ferrous myoglobin or as carbon monoxide ferrous myoglobin. The method is applicable to cardiac, smooth, or skeletal muscle from mammals, reptiles, birds, and teleost fish, but failed with the one amphibian and the one shark tested. © 1979.

引用

页码：473 / 481

页数：9

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