A SENSITIVE, RADIOMETRIC ASSAY FOR LYSOPHOSPHATIDYLCHOLINE

被引：10

作者：

DOBMEYER, DJ ^{[1
]}

CORR, PB ^{[1
]}

CREER, MH ^{[1
]}

机构：

[1] WASHINGTON UNIV,SCH MED,DEPT PHARMACOL,ST LOUIS,MO 63110

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 185卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90251-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To facilitate investigation of the metabolism of lysophosphatidylcholine and choline lysoplasmalogen in small quantities of tissue, a method for the quantification of these phospholipid species that is capable of accurate and reproducible analysis in samples which contain less than 1 nmol of total choline lysophospholipid was developed. The procedure employs chloroform and methanol extraction of phospholipids from isolated tissue with subsequent separation of the choline lysophospholipid fraction by high-performance liquid chromatography. The choline lysophospholipids are then acetylated with [3H]acetic anhydride and the [3H]acetyl-lysophosphatidylcholine product is isolated by thin-layer chromatography and quantified by liquid scintillation counting. The choline lysophospholipid content in the sample is determined from a standard curve constructed from samples containing a known amount of synthetic lysophosphatidylcholine with correction for recovery based on the inclusion of [14C]lysophosphatidylcholine as an internal standard. © 1990.

引用

页码：36 / 43

页数：8

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