LYMPHOKINE PRODUCTION IN MOUSE MIXED LYMPHOCYTE-REACTION (MLR) .2. TENTATIVE MAPPING OF MURINE ALLOANTIGENS ACTIVATING MIGRATION-INHIBITION FACTOR AND INTERFERON RELEASE AND THEIR RELATIONSHIP WITH THOSE ACTIVATING PROLIFERATIVE RESPONSE

We have investigated alloantigen differences which stimulate lymphokine release and3H-TdR uptake in primary 'one-way' MLC among allogeneic mice. When mice differing at the whole H-2 region were tested, MIF and immune IF release was observed, along with a marked3H-TdR uptake. Differences at K, D, or I-S-G regions stimulate both lymphokine release and3H-TdR uptake, though stronger immune IF and3H-TdR responses were observed with differences at I-S-G regions. On the other hand, when mice differing in their minor histocompatibility antigens, and notably at the Mls locus, were tested, lymphokine release took place even in the absence of proliferation. Lastly, in MLC between mice differing at multiple minor loci, but H-2 and Mls matched, MIF release only, and not immune IF and3H-TdR responses were observed in a few combinations. These findings show that T lymphocytes can recognize alloantigens by releasing lymphokines even without going through proliferation. Moreover, different levels of T-lymphocyte activation exist, depending on the kind of stimulating alloantigens present. © 1979 Springer-Verlag New York Inc.