RAPID PURIFICATION OF PROTEIN PHOSPHATASE-2A FROM MOUSE-BRAIN BY MICROCYSTIN-AFFINITY CHROMATOGRAPHY

被引：30

作者：

NISHIWAKI, S ^{[1
]}

FUJIKI, H ^{[1
]}

SUGANUMA, M ^{[1
]}

NISHIWAKIMATSUSHIMA, R ^{[1
]}

SUGIMURA, T ^{[1
]}

机构：

[1] NATL CANC CTR,RES INST,DIV CANC PREVENT,TSUKIJI 5-1-1,CHUO KU,TOKYO 104,JAPAN

来源：

FEBS LETTERS | 1991年 / 279卷 / 01期

关键词：

PROTEIN PHOSPHATASE-2A; MICROCYSTIN; AFFINITY CHROMATOGRAPHY;

D O I：

10.1016/0014-5793(91)80264-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Microcystin LR, which is a monocyclic heptapeptide containing two L-amino acids, leucine and arginine, is a new inhibitor of protein phosphatases 1 and 2A. Microcystin LR-affinity chromatography was used to purify protein phosphatase 2A as a holoenzyme. Five mg of microcystin LR were immobilized to ECH Sepharose 4B by the carbodiimide coupling reaction. Following DEAE-cellulose column chromatography, microcystin-affinity chromatography, as the second step in the procedure, resulted in purification of protein phosphatase 2A in a pure form. The enzyme isolated from mouse brain consisted of two regulatory subunits of 67 kDa and 58 kDa and a catalytic subunit of 41 kDa. Microcystin-affinity chromatography is useful for isolation of protein phosphatase 2A.

引用

页码：115 / 118

页数：4

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