RAPID PURIFICATION OF PROTEIN PHOSPHATASE-2A FROM MOUSE-BRAIN BY MICROCYSTIN-AFFINITY CHROMATOGRAPHY

被引:30
作者
NISHIWAKI, S [1 ]
FUJIKI, H [1 ]
SUGANUMA, M [1 ]
NISHIWAKIMATSUSHIMA, R [1 ]
SUGIMURA, T [1 ]
机构
[1] NATL CANC CTR,RES INST,DIV CANC PREVENT,TSUKIJI 5-1-1,CHUO KU,TOKYO 104,JAPAN
关键词
PROTEIN PHOSPHATASE-2A; MICROCYSTIN; AFFINITY CHROMATOGRAPHY;
D O I
10.1016/0014-5793(91)80264-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Microcystin LR, which is a monocyclic heptapeptide containing two L-amino acids, leucine and arginine, is a new inhibitor of protein phosphatases 1 and 2A. Microcystin LR-affinity chromatography was used to purify protein phosphatase 2A as a holoenzyme. Five mg of microcystin LR were immobilized to ECH Sepharose 4B by the carbodiimide coupling reaction. Following DEAE-cellulose column chromatography, microcystin-affinity chromatography, as the second step in the procedure, resulted in purification of protein phosphatase 2A in a pure form. The enzyme isolated from mouse brain consisted of two regulatory subunits of 67 kDa and 58 kDa and a catalytic subunit of 41 kDa. Microcystin-affinity chromatography is useful for isolation of protein phosphatase 2A.
引用
收藏
页码:115 / 118
页数:4
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