ISOLATION OF A CHROMOID FROM THE CHLOROPLAST OF CHLAMYDOMONAS-REINHARDI

Chloroplast preparations have been obtained from Chlamydomonas reinhardi mutant CW15 which lacks cell walls. The chloroplast suspensions were largely free of nuclear contamination but the organelles retained their capacity of incorporating thymidine and uracil precursors, in DNA and RNA, respectively, thus assessing the presence of the corresponding pathways. The chromoid was obtained by lysis of the chloroplast and purified by gel filtration. The transcriptional activity of the material was effective without the addition of any exogenous polymerase. © 1979.