BIOLOGICAL-ACTIVITIES OF GLYCOPROTEINS OF HVJ (SENDAI VIRUS) STUDIED BY RECONSTITUTION OF HYBRID ENVELOPE AND BY CONCANAVALIN A-MEDIATED BINDING - NEW FUNCTION OF HANA PROTEIN AND STRUCTURAL REQUIREMENT FOR F-PROTEIN IN HEMOLYSIS

When HANA protein of HVJ (Sendai virus) was denatured by dithiothreitol treatment, concanavalin A added to the system or influenza virus hemagglutinin reconstituted with treated HVJ-envelope to form hybrid by SM-2 dialysis method could not replace a role of HANA for hemolysis. Binding of the treated samples to erythrocytes was restored by them, however. Thus, a hypothesis is proposed that intactness of not only F but also HANA protein of HVJ is required for envelope-cell fusion, in other words, HANA protein of HVJ may play some positive role other than binding in the process of the virus-induced hemolysis. © 1979.