ANALYSIS OF QUATERNARY AMMONIUM-COMPOUNDS AND BASIC DRUGS BASED ON ION-PAIR ADSORPTION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A general approach to the ion-pair adsorption high-performance liquid chromatography of basic drugs and quaternary ammonium compounds ins decribed in which suitable counter ions such as Br- and ClO4- are dissolved in the eluent. The columns are packed with silica (e.g., Lichrosorb and LiChrosfer). The systems thus obtained showed high efficiencies and stability in which th ecapacity ratios were found to be exponentially dependent on the concentration of the counter ion. This indicates that the separation mechanism is dominated by adsorption processes. The degree of retention and the separation order can be varied by the nature and the concentration of the counter ion, the sorbent and the composition of the eluent. © 1979.