EVALUATION OF 2 FLUORESCENT TEST KITS FOR DETECTION OF SELECTED BACTEROIDES SPECIES IN CLINICAL SPECIMENS

被引:7
作者
HOLST, E
OSCARSON, J
MARDH, PA
机构
[1] UNIV LUND,INST MED MICROBIOL,S-22362 LUND,SWEDEN
[2] UNIV LUND HOSP,DEPT SURG,S-22185 LUND,SWEDEN
关键词
D O I
10.1007/BF02601854
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Two pools of isothiocyanate fluorescein-conjugated rabbit antisera to Bacteroides fragilis, B. thetaiotaomicron, B. vulgatus, B. distasonis, B. ovatus, and to B. melaninogenicus, subsp. melaninogenicus and intermedius, and B. asaccharolyticus, respectively, were used in immunofluorescent (IF) tests of smears of 122 clinical specimens from a variety of sources, including abscesses and wounds. Smears of broth medium of 14 blood cultures were also tested. Bacteria of the species and subspecies mentioned were detected by the IF tests in 100 and by culture studies in 106 of the 136 speciments tested. Results of the culture studies indicated that 6 tests were false-negative by the IF test, while no false-positive tests were found. The sensitivities of the two test kits were 97% and 85%, respectively, while their specificities were 100%. The predicted value for a positive test with any of the two pools of antisera was 100%, while the predicted value for a negative test was 93% and 97%, respectively. The use of the two test kits (Fluoretec F and M) was found to be a valuable means for a rapid diagnosis of human infections with the Bacteroides species mentioned. © 1979 Springer-Verlag New York Inc.
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页码:133 / 136
页数:4
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