OXIDANT-SCAVENGING ACTIVITIES OF AMPICILLIN AND SULBACTAM AND THEIR EFFECTS ON NEUTROPHIL FUNCTIONS

被引:24
作者
GUNTHER, MR
MAO, JH
COHEN, MS
机构
[1] UNIV N CAROLINA,DEPT MED,CHAPEL HILL,NC 27599
[2] UNIV N CAROLINA,DEPT MICROBIOL,CHAPEL HILL,NC 27599
[3] UNIV N CAROLINA,DEPT IMMUNOL,CHAPEL HILL,NC 27599
关键词
D O I
10.1128/AAC.37.5.950
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Luminol-enhanced luminescence is a method used to measure formation of reactive oxygen intermediates important in the ability of neutrophils to kill microbes. Several studies have demonstrated that under some conditions of incubation, ampicillin can inhibit neutrophil-derived luminol-enhanced luminescence. We evaluated the mechanism(s) by which ampicillin inhibited the luminescent response of stimulated neutrophils. We also investigated sulbactam, a beta-lactamase inhibitor which has been given in combination with ampicillin and other beta-lactam antibiotics to increase their spectra, for possible similar effects. Both ampicillin and sulbactam attenuated luminol-enhanced luminescence by approximately 40%. Superoxide production was not prevented by added ampicillin, nor was superoxide scavenged by it. Myeloperoxidase reacts with H2O2 and Cl-to generate OCl-, which is believed to be the oxidizer of luminol that is primarily responsible for enhancement of neutrophil-derived luminescence. Hydroxyl radicals (HO.), which mrv also oxidize luminol, resulting in luminescence, can be formed from O2- and H2O2 via either myeloperoxidase-dependent (involving intermediate OCl-) or myeloperoxidase-independent (through a metal ion catalyst) reactions. Ampicillin scavenged H2O2 and OCl- and prevented 95% of Fenton reaction-generated HO. from reacting with 5,5-dimethyl-1-pyrroline-N-oxide. Sulbactam was found to scavenge OCl- and HO., but less avidly than ampicillin did. Neither ampicillin nor sulbactam inhibited myeloperoxidase activity. Sublethal concentrations of sulbactam had no significant effect on neutrophil killing of Stapkylococcus aureus and Escherichia coli. Our results demonstrate a mechanism(s) by which ampicillin inhibits luminol-enhanced luminescence from stimulated neutrophils, namely, through scavenging of the oxidant(s) primarily responsible for the generation of luminescence.
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页码:950 / 956
页数:7
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