Dermacentor andersoni: Salivary gland proteins suppressing T-lymphocyte responses to concanavalin A in vitro

被引：41

作者：

Bergman, DK ^{[1
]}

Ramachandra, RN ^{[1
]}

Wikel, SK ^{[1
]}

机构：

[1] OKLAHOMA STATE UNIV, DEPT ENTOMOL, STILLWATER, OK 74078 USA

来源：

EXPERIMENTAL PARASITOLOGY | 1995年 / 81卷 / 03期

关键词：

Concanavalin A (ConA); counts per minute (cpm); Dermacentor andersoni; Escherichia coli; lipopolysaccharide (LPS); fetal bovine serum (FBS); immunosuppression; interleukin-1 (IL-1); interleukin-2 (IL-2); interferon gamma (IFN-G); kilodalton (kDa); octylthioglucoside (OTG); 18 mM monobasic potassium phosphate; 57 mM dibasic sodium phosphate; 75 mM sodium chloride; pH; 7.2; (PBS); phytohemagglutinin (PHA); Roswell Park Memorial Institute medium 1640 (RPMI 1640); salivary glands; sodium dodecyl sulfate (SDS); sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) standard error (SE); T-lymphocytes; tick; tumor necrosis factor alpha (TNF);

D O I：

10.1006/expr.1995.1117

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Salivary glands obtained from feeding adult female Dermacentor andersoni (Acari:Ixodidae) were fractionated using differential centrifugation, detergents, centrifugal concentrators incorporating filter membranes with various molecular weight cutoffs, and preparative SDS-PAGE. A lymphocyte proliferation assay was used to evaluate the effects of salivary gland fractions on ConA-induced blastogenesis of normal murine splenocytes. Lipid, soluble, and detergent-soluble fractions were found to significantly suppress ConA-induced proliferation of splenocytes. Fractions containing soluble proteins suppressed splenocyte proliferation by ca. 26%. Suppressant activity in these fractions was due to components with molecular weights greater than 30 kDa. This suppression of splenocyte proliferation occurred with as little as 0.25 mu g protein per well. Salivary,oland preparative SDS-PAGE fractions containing one or more soluble polypeptides or proteins with molecular weights in the range 36 to 43 kDa significantly suppressed murine splenocyte responses to ConA in vitro. (C) 1995 Academic Press, Inc.

引用

页码：262 / 271

页数：10

共 24 条

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